In the face of accelerating biodiversity loss, the transport sector and associated linear infrastructure developments have a critical role to play in promoting environmental sustainability through targeted nature restoration efforts. This presentation builds on the IENE Transport4Nature Initiative for integrating nature-positive objectives within the transport industry, emphasising the strategic setting of clear, measurable committments for biodiversity conservation and ecosystem restoration. Consideration of appropriate methodologies for assessing ecological impacts specific to transport projects, e.g. roads and railways, which often present unique challenges due to their extensive spatial footprints and fragmentation effects, and linking these to overarching corporate strategies and targets. This extends to the implementation of science-based targets (e.g. SBTN), that align with international conservation goals (i.e. Global Biodiversity Framework) and voluntary frameworks (e. TNFD), facilitating a balance between infrastructure development and nature recovery. In addition, the significance of cross-sector collaboration, involving policymakers, environmental NGOs, and industry stakeholders, to enhance the efficacy of nature targets is highlighted. The presentation underscores the urgent need for the transport sector to adopt and rigorously pursue nature-positive strategies as part of their core business operations, thereby contributing to a sustainable and ecologically responsible future.